Autologous fat grafting is commonly used as a filler material to correct soft tissue defects of various sizes. Its applications range from reconstructive treatment as well as for cosmetic enhancements, such as breast augmentation, hand and face rejuvenation. Recently, deep investigations were just concentrating on the biological and physiological aspect of fat tissue which has driven the line from being just an autologous material for filling to a multipotent stem cell source for multiple regenerative purposes. After their isolation from harvested fat, adipose derived stem/stromal cells (ASCs) or multipotent stromal-derived cells obtained from Stromal Vascular Fraction (SVF), can give wide possibilities in regenerative medicine approach. They can differentiate not only to adipogenic lineage but also to osteogenic, chond